Title | Detection and Distinguishing Infectious Bursal Disease Virus (IBDV) Strains by Molecular Biology Method |
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Project Leader | |
Name | Abdul Rahman Omar |
Telephone No. | 03-89462102 |
aro@vet.upm.edu.my | |
Faculty | Veterinary Medicine |
IP Information | |
IP Type | Patent |
Filed Date | 09-01-2009 |
Application No. | 12/551,969 |
Country Filing | United State of America |
Applicant | Universiti Putra Malaysia |
Granted Date | 31-01-2007 |
IP Number | 7,858,768 |
Abstract | |
The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. | |
Technology | Virology |
Inventor | Abdul Rahman Omar; Mohd Hair-Bejo; Aini Ideris; Hairul Aini Hamzah |
Statement of Novelty | |
Work Start Date | |
Work End Date | |
Classification | |
Commercialised Status | |
Faculty | Veterinary Medicine |