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Title Detection and Distinguishing Infectious Bursal Disease Virus (IBDV) Strains by Molecular Biology Method
Project Leader
Name Abdul Rahman Omar
Telephone No. 03-89462102
Email aro@vet.upm.edu.my
Faculty Veterinary Medicine
IP Information
IP Type Patent
Filed Date 11-05-2004
Application No. PI20044610
Country Filing Malaysia
Applicant Universiti Putra Malaysia
Granted Date 14-08-2015
IP Number MY143506A
Abstract
The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle(Ct) and melting temperature (Tm) values.
Technology Virology
Inventor Abdul Rahman Omar, Mohd Hair Bejo, Aini Ideris, Hairul Aini Hamzah
Statement of Novelty
Work Start Date
Work End Date
Classification
Commercialised Status
Faculty Veterinary Medicine